Journal: Pharmacological research

Article Title: CircRNA CDR1as affects functional repair after spinal cord injury and regulates fibrosis through the SMAD pathway.

doi: 10.1016/j.phrs.2024.107189

Figure Lengend Snippet: Fig. 4. CDR1as regulates NSCs differentiation and microglia polarization. NSCs differentiation was identified by immunofluorescence. GFAP staining was used to detect astrocytes and MAP2 was used to detect nerve cells (A-B). The differentiation was further identified by PCR. The characteristics of nerve cells included MAP2, Nestin and TUJ1, and the characteristics of astrocytes were GFAP (C). iNOS (D) and Arg-1 (E) were detected by immunofluorescence after the BV2 model was established in vitro. Quantitative analysis of immunofluorescence staining (F). Expression of proinflammatory cytokines iNOS, IL-6, TNF-α (G) and anti-inflammatory cytokines Arg-1, CD206 and IL-4 (H) by PCR. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: The immunofluorescence steps of NSCs and BV2 differentiation are consistent with the identification of fibroblast, and primary antibodies were: inducible nitric oxide synthase (iNOS,6022, Proteintech, Rosemont, USA), Arginase-1 (Arg-1, 16001–1-AP, Proteintech), GFAP (3670, CST), and microtubule-associated protein 2 (MAP2, 17490–1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, In Vitro, Expressing

Journal: Journal of molecular and cellular cardiology

Article Title: Myofilament Ca2+ desensitization mediates positive lusitropic effect of neuronal nitric oxide synthase in left ventricular myocytes from murine hypertensive heart.

doi: 10.1016/j.yjmcc.2013.04.017

Figure Lengend Snippet: Fig. 2. Protein expressions of NOS1 and NOS3 and NO production in LV myocytes from shams and Ang II-treated HP rats. A. Representative immunoblottings of NOS1 and NOS3 and their respective GAPDH. B. Averaged NOS1/GAPDH and NOS3/GAPDH ratios in shams and in HP. NOS1 protein level was significantly greater in HP. However, NOS3 protein level was significantly reduced in HP. C. NO production (relative to control) was significantly increased in LV myocytes from HP. Both SMTC and L-VNIO blocked NO production in shams and in HP.

Article Snippet: Immunoblots were performed in rat LV myocyte lysates using specific antibodies against NOS1 (Santa Cruz) and NOS3 (BD Transduction Laboratories), total PLN (Affinity Bioreagents) and its Ser16 phosphorylated fraction (PLN-Ser16, Upstate), total and phosphorylated fraction of cardiac troponin I: cTnI (Santa Cruz) and cTnI23/24 (Cell Signaling), total and phospho-specific cMyBP-C antibodies [18].

Techniques: Control

Journal: Journal of Neurogastroenterology and Motility

Article Title: Molecular and Cellular Characteristics of the Colonic Pseudo-obstruction in Patients With Intractable Constipation

doi: 10.5056/jnm15048

Figure Lengend Snippet: Distribution of neuronal nitric oxide synthase positive (nNOS+) immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.

Article Snippet: Cryostat sections were cut to 20 μm, which were then pre-incubated in 1% bovine serum albumin for 1 hour to reduce non-specific binding and incubated at 4°C overnight with antibodies against neuronal nitric oxide synthase (nNOS) (1:500 dilution; BD Transduction Laboratories, San Jose, CA, USA), vasoactive intestinal peptide (VIP) (1:400 dilution; Millipore, Billerica, MA, USA), vesicular acetylcholine transporter (vAChT) (1:200 dilution; Phoenix Pharmaceuticals, Burlingame, CA, USA) and substance P (SP) (1:400 dilution; Millipore).

Techniques: